Synthesis of amides and peptides using new enzyme function
نویسنده
چکیده
We have extensively studied microbial metabolism of toxic compounds with a triple bond between carbon and nitrogen, such as nitriles [R–CN] . In the Pseudomonas chlororaphis B23 strain, nitrile is hydrated to amide by nitrile hydratase (NHase), followed by degradation to acid by amidase. This strain’s NHase was previously used for the industrial acrylamide production and is now used for the production of 5-cyanovaleramide, we clarified the NHase gene organization composed of seven genes (oxdA, amiA, nhpA, nhpB, nhpC, nhpS and acsA; e,g, OxdA is aldoxime dehydratase; AmiA is amidase; NhpA is NHase alpha-subunit; NhpB is NHase beta-subunit; AcsA is acyl-CoA synthetase).
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